development a new human skin continuous cell line sensitive to mumps virus
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abstract
background and aims: during the last years a new foreskin cell line strain (rfsc) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of hcb (human cell bank) development program in razi institute. materials and methods: the cell population was serially passaged for mass production purpose based on who schedule .the controls on pool samples were carried out upon our sampling method and international recommendations. results: the data concerning parameters as well as the result of compulsory controls on kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed. conclusion: kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line. virus susceptibility shows rfsc-1 more sensitive to rs#12 mumps virus strain. this cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of mumps vaccine.
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Journal title:
iranian journal of virologyجلد ۷، شماره ۴، صفحات ۷-۱۳
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